PoplarV1, Main, Exploration, bibRecord, 000091

Tailoring poplar lignin without yield penalty by combining a null and haploinsufficient CINNAMOYL-CoA REDUCTASE2 allele.

Identifieur interne : 000091 ( Main/Exploration ); précédent : 000090; suivant : 000092

Tailoring poplar lignin without yield penalty by combining a null and haploinsufficient CINNAMOYL-CoA REDUCTASE2 allele.

Auteurs : Barbara De Meester [Belgique] ; Barbara Madariaga Calder N [Belgique] ; Lisanne De Vries [Belgique] ; Jacob Pollier [Belgique] ; Geert Goeminne [Belgique] ; Jan Van Doorsselaere [Belgique] ; Mingjie Chen [États-Unis] ; John Ralph [États-Unis] ; Ruben Vanholme [Belgique] ; Wout Boerjan [Belgique]

Source :

Nature communications [ 2041-1723 ] ; 2020.

RBID : pubmed:33024118

Descripteurs français

KwdFr :
- Aldehyde oxidoreductases (génétique), Aldehyde oxidoreductases (métabolisme), Allèles (MeSH), Haploinsuffisance (MeSH), Lignine (génétique), Lignine (métabolisme), Mutation (MeSH), Populus (croissance et développement), Populus (génétique), Populus (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Techniques de knock-out de gènes (MeSH), Végétaux génétiquement modifiés (MeSH), Xylème (métabolisme), Xylème (ultrastructure).
MESH :
- croissance et développement : Populus.
- génétique : Aldehyde oxidoreductases, Lignine, Populus, Protéines végétales.
- métabolisme : Aldehyde oxidoreductases, Lignine, Populus, Protéines végétales, Xylème.
- ultrastructure : Allèles, Haploinsuffisance, Mutation, Techniques de knock-out de gènes, Végétaux génétiquement modifiés, Xylème.

English descriptors

KwdEn :
- Aldehyde Oxidoreductases (genetics), Aldehyde Oxidoreductases (metabolism), Alleles (MeSH), Gene Knockout Techniques (MeSH), Haploinsufficiency (MeSH), Lignin (genetics), Lignin (metabolism), Mutation (MeSH), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (MeSH), Populus (genetics), Populus (growth & development), Populus (metabolism), Xylem (metabolism), Xylem (ultrastructure).
MESH :
- chemical , genetics : Aldehyde Oxidoreductases, Lignin, Plant Proteins.
- chemical , metabolism : Aldehyde Oxidoreductases, Lignin, Plant Proteins.
- genetics : Populus.
- growth & development : Populus.
- metabolism : Populus, Xylem.
- ultrastructure : Xylem.
- Alleles, Gene Knockout Techniques, Haploinsufficiency, Mutation, Plants, Genetically Modified.

Abstract

Lignin causes lignocellulosic biomass recalcitrance to enzymatic hydrolysis. Engineered low-lignin plants have reduced recalcitrance but often exhibit yield penalties, offsetting their gains in fermentable sugar yield. Here, CRISPR/Cas9-generated CCR2(-/*) line 12 poplars have one knockout CCR2 allele while the other contains a 3-bp deletion, resulting in a 114I115A-to-114T conversion in the corresponding protein. Despite having 10% less lignin, CCR2(-/*) line 12 grows normally. On a plant basis, the saccharification efficiency of CCR2(-/*) line 12 is increased by 25-41%, depending on the pretreatment. Analysis of monoallelic CCR2 knockout lines shows that the reduced lignin amount in CCR2(-/*) line 12 is due to the combination of a null and the specific haploinsufficient CCR2 allele. Analysis of another CCR2(-/*) line shows that depending on the specific CCR2 amino-acid change, lignin amount and growth can be affected to different extents. Our findings open up new possibilities for stably fine-tuning residual gene function in planta.

DOI: 10.1038/s41467-020-18822-w
PubMed: 33024118
PubMed Central: PMC7538556

Affiliations:

Links toward previous steps (curation, corpus...)

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to stream Main, to step Curation: 000068

Le document en format XML

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<author><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aldehyde Oxidoreductases (genetics)</term>
<term>Aldehyde Oxidoreductases (metabolism)</term>
<term>Alleles (MeSH)</term>
<term>Gene Knockout Techniques (MeSH)</term>
<term>Haploinsufficiency (MeSH)</term>
<term>Lignin (genetics)</term>
<term>Lignin (metabolism)</term>
<term>Mutation (MeSH)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Populus (genetics)</term>
<term>Populus (growth & development)</term>
<term>Populus (metabolism)</term>
<term>Xylem (metabolism)</term>
<term>Xylem (ultrastructure)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Aldehyde oxidoreductases (génétique)</term>
<term>Aldehyde oxidoreductases (métabolisme)</term>
<term>Allèles (MeSH)</term>
<term>Haploinsuffisance (MeSH)</term>
<term>Lignine (génétique)</term>
<term>Lignine (métabolisme)</term>
<term>Mutation (MeSH)</term>
<term>Populus (croissance et développement)</term>
<term>Populus (génétique)</term>
<term>Populus (métabolisme)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Techniques de knock-out de gènes (MeSH)</term>
<term>Végétaux génétiquement modifiés (MeSH)</term>
<term>Xylème (métabolisme)</term>
<term>Xylème (ultrastructure)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Aldehyde Oxidoreductases</term>
<term>Lignin</term>
<term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Aldehyde Oxidoreductases</term>
<term>Lignin</term>
<term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Aldehyde oxidoreductases</term>
<term>Lignine</term>
<term>Populus</term>
<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term>
<term>Xylem</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Aldehyde oxidoreductases</term>
<term>Lignine</term>
<term>Populus</term>
<term>Protéines végétales</term>
<term>Xylème</term>
</keywords>
<keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Xylem</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Alleles</term>
<term>Gene Knockout Techniques</term>
<term>Haploinsufficiency</term>
<term>Mutation</term>
<term>Plants, Genetically Modified</term>
</keywords>
<keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Allèles</term>
<term>Haploinsuffisance</term>
<term>Mutation</term>
<term>Techniques de knock-out de gènes</term>
<term>Végétaux génétiquement modifiés</term>
<term>Xylème</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Lignin causes lignocellulosic biomass recalcitrance to enzymatic hydrolysis. Engineered low-lignin plants have reduced recalcitrance but often exhibit yield penalties, offsetting their gains in fermentable sugar yield. Here, CRISPR/Cas9-generated CCR2(-/*) line 12 poplars have one knockout CCR2 allele while the other contains a 3-bp deletion, resulting in a 114I115A-to-114T conversion in the corresponding protein. Despite having 10% less lignin, CCR2(-/*) line 12 grows normally. On a plant basis, the saccharification efficiency of CCR2(-/*) line 12 is increased by 25-41%, depending on the pretreatment. Analysis of monoallelic CCR2 knockout lines shows that the reduced lignin amount in CCR2(-/*) line 12 is due to the combination of a null and the specific haploinsufficient CCR2 allele. Analysis of another CCR2(-/*) line shows that depending on the specific CCR2 amino-acid change, lignin amount and growth can be affected to different extents. Our findings open up new possibilities for stably fine-tuning residual gene function in planta.</div>
</front>
</TEI>
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<DateCompleted><Year>2020</Year>
<Month>10</Month>
<Day>29</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>10</Month>
<Day>29</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2041-1723</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>11</Volume>
<Issue>1</Issue>
<PubDate><Year>2020</Year>
<Month>10</Month>
<Day>06</Day>
</PubDate>
</JournalIssue>
<Title>Nature communications</Title>
<ISOAbbreviation>Nat Commun</ISOAbbreviation>
</Journal>
<ArticleTitle>Tailoring poplar lignin without yield penalty by combining a null and haploinsufficient CINNAMOYL-CoA REDUCTASE2 allele.</ArticleTitle>
<Pagination><MedlinePgn>5020</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1038/s41467-020-18822-w</ELocationID>
<Abstract><AbstractText>Lignin causes lignocellulosic biomass recalcitrance to enzymatic hydrolysis. Engineered low-lignin plants have reduced recalcitrance but often exhibit yield penalties, offsetting their gains in fermentable sugar yield. Here, CRISPR/Cas9-generated CCR2(-/*) line 12 poplars have one knockout CCR2 allele while the other contains a 3-bp deletion, resulting in a 114I115A-to-114T conversion in the corresponding protein. Despite having 10% less lignin, CCR2(-/*) line 12 grows normally. On a plant basis, the saccharification efficiency of CCR2(-/*) line 12 is increased by 25-41%, depending on the pretreatment. Analysis of monoallelic CCR2 knockout lines shows that the reduced lignin amount in CCR2(-/*) line 12 is due to the combination of a null and the specific haploinsufficient CCR2 allele. Analysis of another CCR2(-/*) line shows that depending on the specific CCR2 amino-acid change, lignin amount and growth can be affected to different extents. Our findings open up new possibilities for stably fine-tuning residual gene function in planta.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>De Meester</LastName>
<ForeName>Barbara</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052, Ghent, Belgium.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>VIB Center for Plant Systems Biology, Technologiepark 71, 9052, Ghent, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Madariaga Calderón</LastName>
<ForeName>Barbara</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052, Ghent, Belgium.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>VIB Center for Plant Systems Biology, Technologiepark 71, 9052, Ghent, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>de Vries</LastName>
<ForeName>Lisanne</ForeName>
<Initials>L</Initials>
<Identifier Source="ORCID">0000-0002-3245-9081</Identifier>
<AffiliationInfo><Affiliation>Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052, Ghent, Belgium.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>VIB Center for Plant Systems Biology, Technologiepark 71, 9052, Ghent, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Pollier</LastName>
<ForeName>Jacob</ForeName>
<Initials>J</Initials>
<Identifier Source="ORCID">0000-0002-1134-9238</Identifier>
<AffiliationInfo><Affiliation>VIB Metabolomics Core, Technologiepark 71, 9052, Ghent, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Goeminne</LastName>
<ForeName>Geert</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>VIB Metabolomics Core, Technologiepark 71, 9052, Ghent, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Van Doorsselaere</LastName>
<ForeName>Jan</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Higher Institute for Nursing and Biotechnology, VIVES University College, Wilgenstraat 32, 8800, Roeselare, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Mingjie</ForeName>
<Initials>M</Initials>
<Identifier Source="ORCID">0000-0002-7400-5192</Identifier>
<AffiliationInfo><Affiliation>Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>US Department of Energy, Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, Madison, WI, 53726, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ralph</LastName>
<ForeName>John</ForeName>
<Initials>J</Initials>
<Identifier Source="ORCID">0000-0002-6093-4521</Identifier>
<AffiliationInfo><Affiliation>Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>US Department of Energy, Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, Madison, WI, 53726, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Vanholme</LastName>
<ForeName>Ruben</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052, Ghent, Belgium.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>VIB Center for Plant Systems Biology, Technologiepark 71, 9052, Ghent, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Boerjan</LastName>
<ForeName>Wout</ForeName>
<Initials>W</Initials>
<Identifier Source="ORCID">0000-0003-1495-510X</Identifier>
<AffiliationInfo><Affiliation>Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052, Ghent, Belgium. wout.boerjan@psb.vib-ugent.be.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>VIB Center for Plant Systems Biology, Technologiepark 71, 9052, Ghent, Belgium. wout.boerjan@psb.vib-ugent.be.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
<PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2020</Year>
<Month>10</Month>
<Day>06</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Nat Commun</MedlineTA>
<NlmUniqueID>101528555</NlmUniqueID>
<ISSNLinking>2041-1723</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9005-53-2</RegistryNumber>
<NameOfSubstance UI="D008031">Lignin</NameOfSubstance>
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<Chemical><RegistryNumber>EC 1.2.-</RegistryNumber>
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<Chemical><RegistryNumber>EC 1.2.1.44</RegistryNumber>
<NameOfSubstance UI="C019904">cinnamoyl CoA reductase</NameOfSubstance>
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<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000483" MajorTopicYN="N">Alleles</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D055786" MajorTopicYN="N">Gene Knockout Techniques</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D057895" MajorTopicYN="N">Haploinsufficiency</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D052584" MajorTopicYN="N">Xylem</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName>
</MeshHeading>
</MeshHeadingList>
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<tree><country name="Belgique"><region name="Région flamande"><name sortKey="De Meester, Barbara" sort="De Meester, Barbara" uniqKey="De Meester B" first="Barbara" last="De Meester">Barbara De Meester</name>
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<name sortKey="De Vries, Lisanne" sort="De Vries, Lisanne" uniqKey="De Vries L" first="Lisanne" last="De Vries">Lisanne De Vries</name>
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<name sortKey="Madariaga Calder N, Barbara" sort="Madariaga Calder N, Barbara" uniqKey="Madariaga Calder N B" first="Barbara" last="Madariaga Calder N">Barbara Madariaga Calder N</name>
<name sortKey="Pollier, Jacob" sort="Pollier, Jacob" uniqKey="Pollier J" first="Jacob" last="Pollier">Jacob Pollier</name>
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<country name="États-Unis"><noRegion><name sortKey="Chen, Mingjie" sort="Chen, Mingjie" uniqKey="Chen M" first="Mingjie" last="Chen">Mingjie Chen</name>
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Tailoring poplar lignin without yield penalty by combining a null and haploinsufficient CINNAMOYL-CoA REDUCTASE2 allele.

Tailoring poplar lignin without yield penalty by combining a null and haploinsufficient CINNAMOYL-CoA REDUCTASE2 allele.

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